NFIB antibody (10R-1200)

Mouse monoclonal NFIB antibody

Synonyms Monoclonal NFIB antibody, Anti-NFIB antibody, NFIB2 antibody, NFI-RED antibody, Nuclear factor 1 B-type antibody, NFIB3 antibody, NFIB antibody, Nuclear factor 1 B-type antibody, NFIB3 antibody, NFIB2 antibody, Anti NFIB antibody, NFI-RED antibody
Specificity Human NFIB
Cross Reactivity Human. Other species not studied
Applications Dot Blot, IP
Immunogen NFIB antibody was raised in mouse using recombinant Nuclear Factor I/B

Images

Immunofluorescent staining using NFIB antibody (10R-1200)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-NFIB antibody.

Specifications

Host Mouse
Clone NFI5I299
Isotype IgG1
Method of Purification NFIB antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer NFIB antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

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Images

  • Immunofluorescent staining using NFIB antibody (10R-1200) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-NFIB antibody.
  • Western Blot analysis of immunized recombinant protein using NFIB antibody (10R-1200)

Availability: In stock

Price: €364.83
Size: 100 ug
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