NFKB C-rel antibody (20-CR07)
Rabbit polyclonal NFKB C-rel antibody
Overview
Overview
| Synonyms | Polyclonal NFKB C-rel antibody, Anti-NFKB C-rel antibody, NFkappaB antibody, Nuclear factor NF-kappa-B p65 subunit antibody, NFKB antibody, nfkb antibody, NF-kB antibody, NF-kappaB antibody |
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| Specificity | Human NFKB C-rel |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, WB |
| Immunogen | NFKB C-rel antibody was raised in rabbit using NFkB cRel peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH as the immunogen. |
Images
Western Blot analysis using NFKB C-rel antibody (20-CR07)
Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0. 1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1, 000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 mins each. The western blot was incubated with Goat anti-Rabbit IgG (H + L) (HRP) diluted 1:2, 000 for 1 h. Washes with TBS preceded color development.
Specifications
| Host | Rabbit |
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| Method of Purification | NFKB C-rel antibody was purified by delipidation and defibrination. |
| Form & Buffer | 0.02M K3PO4,pH 7.2, with 0.15M NaCl, and 0.01%NaN3. |
| Concentration | 80.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:5,000-1:25,000, WB: 1:500-1:3,000 |
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Storage & Safety
| Storage | Store at -20 deg C. Aliquot and freeze at -20 deg C or below for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NF kb1) subunits. |
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