NFKB p105/p50 antibody (70R-30595)

Rabbit polyclonal NFKB p105/p50 antibody

Synonyms Polyclonal NFKB p105/p50 antibody, Anti-NFKB p105/p50 antibody, DNA-binding factor KBF1 antibody, EBP-1 antibody, NF-kappa-B1 p84/NF-kappa-B1 p98 antibody, NFKB1 antibody, NFkB-p50 antibody, Nuclear factor NF-kappa-B p105 subunit antibody, nuclear factor NF-kappa-B p50 subunit antibody
Specificity NFKB p105/p50 antibody detects endogenous levels of total NF-kappaB p105/p50 protein
Cross Reactivity Mouse
Applications ELISA, IF, IHC, WB
Immunogen NFKB p105/p50 antibody was raised in Rabbit using a synthesized non-phosphopeptide derived from human NF- kappaB p105/p50 around the phosphorylation site of serine Ab-927) 927 (C-D-SP-G-V) as the immunogen

Images

IHC staining using NFKB p105/p50 antibody (70R-30595)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFKB p105/p50 antibody

Specifications

Host Rabbit
Isotype IgG
Form & Buffer Purified IgG in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:40000

Storage & Safety

Storage Store at -20 deg C in small aliquots. Avoid repeated freeze-thaw cycles

General Information

Biological Significance NFKB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus.

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Images

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IHC staining using NFKB p105/p50 antibody (70R-30595)



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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFKB p105/p50 antibody
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Western blot analysis using NFKB p105/p50 antibody (70R-30595)



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Western blot analysis of extracts from Hela cells treated with TNF-alpha (20ng/ml, 5min) using NFKB p105/p50 antibody
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Immunofluorescent staining using NFKB p105/p50 antibody (70R-30595)



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Immunofluorescence analysis of HeLa cells using NFKB p105/p50 antibody

Availability: In stock

Price: €230.67
Size: 100 ug
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