NFKB P65 Rel A antibody (20R-NR007)
Rabbit polyclonal NFKB P65 Rel A antibody
Overview
Overview
| Synonyms | Polyclonal NFKB P65 Rel A antibody, Anti-NFKB P65 Rel A antibody, Nuclear factor NF-kappa-B p65 subunit antibody, nfkb antibody, NF-kappaB antibody, NFkappaB antibody, NF-kB antibody, NFKB antibody |
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| Specificity | Human NFKB p65 |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, WB |
| Immunogen | NFKB P65 rel A antibody was raised in rabbit using NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH as the immunogen. |
Images
Western blot analysis using NFKB P65 Rel A antibody (20R-NR007)
Western Blot of NFKB p65 antibody. Lane 1-4: PC3 cell lysate. Load: 20, 30, 40, and 50 ug per lane. NFKB p65 antibody at 1:5000 for overnight at 4 deg C. HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: ~65kDa for NFKB p65. Other band(s): ~110kDa and ~45kDa.
Specifications
| Host | Rabbit |
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| Method of Purification | NFKB P65 Rel A antibody was purified by delipidation and defibrination. |
| Form & Buffer | Supplied as a liquid with 0.01% NaN3. |
| Concentration | 90.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:5,000-1:25,000, WB: 1:500-1:3,000 |
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Storage & Safety
| Storage | Store at -20 deg C. Aliquot and freeze at -20 deg C or below for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NF kb1) subunits. |
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