NFKB P65 Rel A antibody (20R-NR008)
Rabbit polyclonal NFKB P65 Rel A antibody
Overview
Overview
| Synonyms | Polyclonal NFKB P65 Rel A antibody, Anti-NFKB P65 Rel A antibody, nfkb antibody, Nuclear factor NF-kappa-B p65 subunit antibody, NFKB antibody, NFkappaB antibody, NF-kB antibody, NF-kappaB antibody |
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| Specificity | Human NFKB p65 |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, Gel Shift, WB |
| Immunogen | NFKB P65 rel A antibody was raised in rabbit using NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH as the immunogen. |
Images
Immunohistochemical staining using NFKB P65 Rel A antibody (20R-NR008)
Immunohistochemistry of ABCB5 antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: ABCB5 1:200. Secondary antibody: Peroxidase goat anti-rabbit at 1:10, 000 for 45 min at RT. Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Specifications
| Host | Rabbit |
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| Method of Purification | NFKB P65 Rel A antibody was purified by delipidation and defibrination. |
| Form & Buffer | Supplied as a liquid with 0.01% NaN3. |
| Concentration | 80 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:5,000, WB: 1:2,000-1:5,000 |
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Storage & Safety
| Storage | Store at -20 deg C. Aliquot and freeze at -20 deg C or below for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NF kb1) subunits. |
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