NIP7 protein (His tag) (80R-1309)
Purified recombinant Human NIP7 protein
|Synonyms||HSPC031 protein, Nuclear import 7 protein, NIP-7, 60S ribosome subunit biogenesis protein NIP7 homolog 60S ribosome subunit biogenesis protein NIP7 homolog protein, cerevisiae) protein, KD93 protein, CGI37 protein, NIP-7 protein, NIP 7 protein, NIP 7, CGI 37 protein, OK/SW cl.76 protein, CGI-37 protein, Nuclear import 7 homolog (S protein, FLJ10296 protein, KD93 protein, HSPC180 protein, NIP 7 protein, NIP7, OK/SW cl.78.|
Coomassie Blue stained SDS-PAGE of NIP7 protein (His tag) (80R-1309)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-180 amino acids: MRPLTEEETR VMFEKIAKYI GENLQLLVDR PDGTYCFRLH NDRVYYVSEK IMKLAANISG DKLVSLGTCFGKFTKTHKFR LHVTALDYLA PYAKYKVWIK PGAEQSFLYG NHVLKSGLGR ITENTSQYQG VVVYSMADIPLGFGVAAKST QDCRKVDPMA IVVFHQADIG EYVRHEETLT LEHHHHHH|
|Grade & Purity||> 90% pure|
|Molecular Weight||21.5 kDa (188aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NIP7, also known as the 60S ribosome subunit biogenesis protein NIP7 homolog, belongs to NIP7 family and contains 1 PUA domain. It interacts with pre-ribosome complex and may bind to RNA. This protein is required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly. Recombinant human NIP7 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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