NIT2 protein (His tag) (80R-1580)

Purified recombinant Human NIT2 protein

Synonyms Omega-amidase NIT2 protein, NIT2, NIT-2 protein, NIT 2, Nitrilase homolog 2, NIT 2 protein, NIT-2
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of NIT2 protein (His tag) (80R-1580)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-276 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMTSFRLA LIQLQISSIK SDNVTRACSF IREAATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQ KLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTFDTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELLQRSRAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAIVYSDID LKKLAEIRQQ IPVFRQKRSD LYAVEMKKP
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 33 kDa (299aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol and 0.1M NaCl.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Omega-amidase NIT2, also known NIT2, belongs to the nitrilase superfamily. This protein has an omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Also Nit2, which is widely distributed in nature, has been suggested to be a tumor suppressor protein. Recombinant human NIT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of NIT2 protein (His tag) (80R-1580) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €319.89
Size: 50 ug
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