Nitrocefin substrate (42R-1003)

Nitrocefin substrate for use in the research laboratory

Synonyms (6R 7R)-3-[2-(2 4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, Nitrocefin substrate, Nitrocefin chromogenic substrate, Nitrocefin beta lactamase substrate

Specifications

Grade & Purity > 95% pure
Molecular Weight 516.5
Form & Buffer Solubility: DMSO

Storage & Safety

Storage Store at -20 deg C, protect from light and air

General Information

Biological Significance Nitrocefin is the chromogenic cephalosporin that acts as an excellent beta-lactamase substrate. It exhibits a rapid distinctive color change from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm) as the amide bond in the beta-lactam ring is hydrolyzed by a beta-lactamase. It is sensitive to hydrolysis by all known lactamases produced by Gram-positive and Gram-negative bacteria.

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Availability: In stock

Price: €92.43
Size: 5 mg
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