NLK antibody (70R-13123)

Affinity purified Rabbit polyclonal NLK antibody

Synonyms Polyclonal NLK antibody, Anti-NLK antibody, serine/threonine-protein kinase NLK antibody, NLK antibody, nemo-like kinase antibody, DKFZp761G1211 antibody, FLJ21033 antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, WB
Immunogen NLK antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 234 and 490 of NLK

Images

Western blot analysis using NLK antibody (70R-13123)

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and NLK antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification NLK antibody was purified by antigen-affinity chromatography
Molecular Weight 58 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using NLK antibody (70R-13123)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and NLK antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Western blot analysis using NLK antibody (70R-13123)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5 % SDS PAGE gel and NLK antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using NLK antibody (70R-13123)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NLK antibody at 1:100 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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