Nme2 protein (80R-4295)
Purified Recombinant Nme2 protein (His tagged)
Overview
Overview
| Synonyms | NDKB protein, nm23-2 protein, p18-12d protein |
|---|---|
| Species | Rat |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
SDS-PAGE analysis using Nme2 protein (His tag) (80R-4295)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-152 aa: MGSSHHHHHH SSGLVPRGSH MGSMANLERT FIAIKPDGVQ RGLVGEIIKR FEQKGFRLVA MKFLRASEEH LKQHYIDLKD RPFFPGLVKY MNSGPVVAMV WEGLNVVKTG RVMLGETNPA DSKPGTIRGD FCIQVGRNII HGSDSVESAE KEIGLWFKPE ELIDYKSCAH DWVYE |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 19.7 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in PBS buffer pH 7.4 contaning 10% glycerol |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage. For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | Nme2 also known as nucleoside diphosphate kinaseB is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. Nme2 is a ubiquitous enzyme that catalyses phosphorylation of nucleoside 5´-diphosphate (NDP) to the corresponding triphosphate (NTP), following a Ping-Pong mechanism which includes the formation of a phosphohistidine intermediate. Recombinant rat Nme2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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