NOTCH1 antibody (10R-5027)

Mouse monoclonal NOTCH1 antibody

Synonyms Monoclonal NOTCH1 antibody, Anti-NOTCH1 antibody, NOTCH-1 antibody, NOTCH 1 antibody, TAN1 antibody, Translocation-associated notch protein TAN-1 antibody, hN1 antibody, Notch homolog 1 antibody, NOTCH1, NOTCH 1, NOTCH-1, NOTCH 1 antibody, NOTCH-1 antibody
Cross Reactivity Human
Applications IF, IHC, RPPA, WB
Immunogen NOTCH1 antibody was raised in mouse using a recombinant protein expressed in E. coli corresponding to amino acids 2300-2556 of human NOTCH1 as the immunogen.

Images

Immunofluorescent staining using NOTCH1 antibody (10R-5027)

Immunofluorescent staining of Hela cells transiently transfected with recombinant NOTCH1 protein using NOTCH1 antibody

Specifications

Host Mouse
Clone 3 E12
Isotype IgG2b
Method of Purification NOTCH1 antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:100, IHC: 1:50, WB: 1:1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance NOTCH1 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is anevolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacentcells.

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Images

  • Immunofluorescent staining using NOTCH1 antibody (10R-5027) | Immunofluorescent staining of Hela cells transiently transfected with recombinant NOTCH1 protein using NOTCH1 antibody
  • Western Blot analysis using NOTCH1 antibody (10R-5027) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NOTCH1 protein (Right) or empty vector (Left) detected with NOTCH1 antibody
  • Immunohistochemical staining using NOTCH1 antibody (10R-5027) | Immunohistochemical analysis of NOTCH1 protein in paraffin embedded Adenocarcinoma of Human breast tissue using NOTCH1 antibody

Availability: In stock

Price: €527.61
Size: 100 ul
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