NOTCH1 antibody was raised in mouse using a recombinant protein expressed in E. coli corresponding to amino acids 2300-2556 of human NOTCH1 as the immunogen.
Immunofluorescent staining using NOTCH1 antibody (10R-5027)
Immunofluorescent staining of Hela cells transiently transfected with recombinant NOTCH1 protein using NOTCH1 antibody
NOTCH1 antibody
Immunofluorescent staining using NOTCH1 antibody (10R-5027)
Immunofluorescent staining of Hela cells transiently transfected with recombinant NOTCH1 protein using NOTCH1 antibody
Western Blot analysis using NOTCH1 antibody (10R-5027)
Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NOTCH1 protein (Right) or empty vector (Left) detected with NOTCH1 antibody
Immunohistochemical staining using NOTCH1 antibody (10R-5027)
Immunohistochemical analysis of NOTCH1 protein in paraffin embedded Adenocarcinoma of Human breast tissue using NOTCH1 antibody
Specifications
Host
Mouse
Clone
3 E12
Isotype
IgG2b
Method of Purification
NOTCH1 antibody was purified by affinity chromatography.
Form & Buffer
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
NOTCH1 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is anevolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacentcells.