NPM antibody (Thr199) (70R-31496)

Rabbit polyclonal NPM antibody (Thr199)

Synonyms Polyclonal NPM antibody, Anti-NPM antibody, NO38 antibody, Nucleolar phosphoprotein B23 antibody, B23 antibody, Nucleolar protein NO38 antibody, NPM1 antibody, MGC104254 antibody, numatrin antibody, Nucleophosmin antibody
Specificity NPM antibody detects endogenous levels of NPM only when phosphorylated at threonine 199
Cross Reactivity Mouse, Rat
Applications ELISA, IF, IHC, WB
Immunogen NPM antibody was raised in Rabbit using a synthesized phosphopeptide derived from human NPM around the phosphorylation site of threonine 199 (R-D-TP-P-A) as the immunogen

Images

IHC staining using NPM antibody (Thr199) (70R-31496)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NPM antibody (Thr199)

Specifications

Host Rabbit
Isotype IgG
Residues Human: Thr199, Mouse: Thr198, Rat: Thr198
Form & Buffer Purified IgG in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:1000, IF: 1:100-1:500, IHC: 1:50-1:100, ELISA: 1:60000

Storage & Safety

Storage Store at -20 deg C in small aliquots. Avoid repeated freeze-thaw cycles

General Information

Biological Significance NPM is a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway.

References
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Images

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IHC staining using NPM antibody (Thr199) (70R-31496)



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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NPM antibody (Thr199)
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Western blot analysis using NPM antibody (Thr199) (70R-31496)



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Western blot analysis of extracts from Jurkat cells using NPM antibody (Thr199)
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Immunofluorescent staining using NPM antibody (Thr199) (70R-31496)



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Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins) using NPM antibody (Thr199)

Availability: In stock

Price: €230.67
Size: 100 ug
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