NR0B1 antibody (70R-12882)

Affinity purified Rabbit polyclonal NR0B1 antibody

Synonyms Polyclonal NR0B1 antibody, Anti-NR0B1 antibody, NROB1 antibody, DAX-1 antibody, nuclear receptor DAX1 antibody, NR0B1 antibody, GTD antibody, AHCH antibody, AHC antibody, DSS antibody, nuclear hormone receptor antibody, DAX1 antibody, AHX antibody, nuclear receptor subfamily 0 group B member 1 antibody, HHG antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen NR0B1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 96 and 406 of NR0B1

Images

Immunofluorescent staining using NR0B1 antibody (70R-12882)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAX-1 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification NR0B1 antibody was purified by antigen-affinity chromatography
Molecular Weight 52 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.4 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, ICC:1:100-1:200, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using NR0B1 antibody (70R-12882)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAX-1 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using NR0B1 antibody (70R-12882)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: HeLa S3) using a 7.5 % SDS PAGE gel and NR0B1 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Immunohistochemical staining using NR0B1 antibody (70R-12882)</font></div>     | Immunohistochemical staining of paraffin-embedded Cal27 xenograft using NR0B1 antibody at a dilution of 1:100

Availability: In stock

Price: €323.11
Size: 100 ul
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