NRAS protein (30R-1492)
Purified recombinant Human NRAS protein
|Synonyms||N ras protein part 4 protein, HRAS1 protein, v ras neuroblastoma RAS viral oncogene homolog, NS6 protein, OTTHUMP00000013879 protein, Neuroblastoma RAS viral (v ras) oncogene homolog protein, ALPS4 protein, GTPase NRas protein, AV095280 protein, N ras protein, Transforming protein N Ras protein, N-ras protein, NRAS1 protein, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMUSP00000023521 protein|
Coomassie Blue stained SDS-PAGE of NRAS protein (30R-1492)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-186 amino acids: MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ GVEDAFYTLV REIRQYRMKK LNSSDDGTQG CMGLPC|
|Grade & Purity||> 95% pure|
|Molecular Weight||20.8 kDa (186aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 100mM NaCl, 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Neuroblastoma RAS viral (v-ras) oncogene homolog, also known as NRAS, is a member of the RAS gene family. NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Recombinant human NRAS was expressed in E. coli and purified by using conventional chromatography techniques.|
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