NSE antibody (20R-3000)
Rabbit polyclonal NSE antibody
Overview
Overview
| Synonyms | Polyclonal NSE antibody, Anti-NSE antibody, ENO2 antibody, enolase 2 (gamma antibody, neuronal) antibody, 2-phospho-D-glycerate hydrolyase antibody, 2-phospho-D-glycerate hydro-lyase antibody, BOS_5972 antibody, D6Ertd375e antibody, DKEY-89B17.1 antibody, EGK_03296 antibody, Eno-2 antibody, eno3 antibody, enolase 2 antibody, enolase 2 antibody, gamma neuronal antibody, enolase 2 antibody, gamma antibody, neuronal antibody, fc09h05 antibody, Gamma-enolase antibody, gamma-subunit of enolase antibody, neural enolase antibody, neurone-specific enolase antibody, neuron-specific enolase antibody, neuron specific gamma enolase antibody, NSE antibody, PANDA_011244 antibody, RNEN3 antibody |
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| Cross Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Immunogen | NSE antibody was raised in Rabbit using a His-tagged recombinant human gamma enolase purified from E.coli as the immunogen |
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | NSE antibody was purified by Protein A affinity chromatography |
| Form & Buffer | Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol |
Usage & Assay Information
| Usage Recommendations | WB: 1:2000 |
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Storage & Safety
| Storage | Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles |
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General Information
| Biological Significance | NSE (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phospho-enolpyruvate. It comprises three distint subunits, alpha, beta and gamma. The gamma-gamma and alpha-gamma dimeric forms of enolase, referred to as neuron-specific enolase (NSE), are localized mainly in neurons and neuroectodermal tissue. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. |
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