NSE antibody (70R-10633)

Affinity purified Chicken polyclonal NSE antibody

Synonyms Polyclonal NSE antibody, Anti-NSE antibody, Neuron specific gamma enolase antibody, Neuron Specific Enolase antibody, Enolase 2 gamma neuronal antibody, gamma-enolase antibody, Enolase 2 antibody, gamma enolase antibody, Neuron specific enolase antibody, Enolase2 antibody, Neural enolase antibody
Applications ICC, IHC, WB
Immunogen NSE antibody was raised in Chicken using two synthetic peptide (Human NP_001966 and Rat AAA41119) / keyhole limpet hemocyanin (KLH) conjugates corresponding to different regions of the NSE-2 gene product as the immunogen.

Images

Immunofluorescent staining of NSE antibody (70R-10633)

Dissociated cell cultures of neonatal mouse brains, showing NSE-2 (green staining) in neurons. These cultures were counter-stained with a rabbit anti-GFAP to localize astrocytes, as well as with DAPI (blue staining) to localize nuclei.

Specifications

Host Chicken
Method of Purification NSE antibody was purified by affinity chromatography.
Form & Buffer Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative.
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 1:2000-1:5000; IHC: 1:2000-1:5000; WB: 1:5000-1:10000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light.

General Information

Biological Significance Neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. NSE is a phosphopyruvate hydratase. NSE is produced by small cell carcinomas which are neuroendocrine in origin. NSE is therefore a useful tumor marker for lung cancer patients.

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Images

  • Immunofluorescent staining of NSE antibody (70R-10633) | Dissociated cell cultures of neonatal mouse brains, showing NSE-2 (green staining) in neurons. These cultures were counter-stained with a rabbit anti-GFAP to localize astrocytes, as well as with DAPI (blue staining) to localize nuclei.

Availability: In stock

Price: €502.25
Size: 1 ml
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