NSE antibody (70R-10633)
Affinity purified Chicken polyclonal NSE antibody
Overview
Overview
| Synonyms | Polyclonal NSE antibody, Anti-NSE antibody, Neuron specific gamma enolase antibody, Neuron Specific Enolase antibody, Enolase 2 gamma neuronal antibody, gamma-enolase antibody, Enolase 2 antibody, gamma enolase antibody, Neuron specific enolase antibody, Enolase2 antibody, Neural enolase antibody |
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| Applications | ICC, IHC, WB |
| Immunogen | NSE antibody was raised in Chicken using two synthetic peptide (Human NP_001966 and Rat AAA41119) / keyhole limpet hemocyanin (KLH) conjugates corresponding to different regions of the NSE-2 gene product as the immunogen. |
Images
Immunofluorescent staining of NSE antibody (70R-10633)
Dissociated cell cultures of neonatal mouse brains, showing NSE-2 (green staining) in neurons. These cultures were counter-stained with a rabbit anti-GFAP to localize astrocytes, as well as with DAPI (blue staining) to localize nuclei.
Specifications
| Host | Chicken |
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| Method of Purification | NSE antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative. |
| Concentration | 200 ug/ml |
Usage & Assay Information
| Usage Recommendations | ICC: 1:2000-1:5000; IHC: 1:2000-1:5000; WB: 1:5000-1:10000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | Neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. NSE is a phosphopyruvate hydratase. NSE is produced by small cell carcinomas which are neuroendocrine in origin. NSE is therefore a useful tumor marker for lung cancer patients. |
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