NSE protein (30R-1165)

Purified recombinant Human NSE protein

Synonyms ENO2 protein, Enolase 2 gamma neuronal protein, neuronal) protein, Neuron specific enolase protein, Neuron specific gamma enolase protein, NSE protein, Gamma enolase protein, 2 phospho D glycerate hydrolyase protein, ENOG protein, Neuron-Specific Enolase protein, Eno 2 protein, Neurone specific enolase., Enolase 2 (gamma protein, Neural enolase protein, Enolase2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of NSE protein (30R-1165)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-434 amino acids: MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 47 kDa ( 434 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris, pH 7.5, containing 0.1 M KCl, and 5 mM MgSO4.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity: > 8 units/ml. One unit will convert 1.0 umole of 2-phosphoglycerate to phospho(enol)pyruvate per minute at pH 7.5 at 25C

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E. coli and purified by conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of NSE protein (30R-1165) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €277.55
Size: 100 ug
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