nth protein (His tag) (80R-1809)
Purified recombinant E.coli nth protein
|Synonyms||DNA-(apurinic or apyrimidinic site) lyase protein, JW1625 protein, Endonuclease III protein, b1633 protein|
Coomassie Blue stained SDS-PAGE of nth protein (His tag) (80R-1809)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-211 amino acids: MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I|
|Grade & Purity||> 85% pure|
|Molecular Weight||25.7 kDa (231aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 40% glycerol, 0.1M NaCl, 1mM DTT and 0.1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Recombinant E. coli nth protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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