NUDT2 protein (His tag) (80R-1648)
Purified recombinant Human NUDT2 protein
|Synonyms||bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] protein, NUDT 2 protein, NUDT2, MGC10404, APAH1 protein, NUDT 2, NUDT-2, NUDT-2 protein|
Coomassie Blue stained SDS-PAGE of NUDT2 protein (His tag) (80R-1648)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-147 amino acids: MGSSHHHHHH SSGLVPRGSH MALRACGLII FRRCLIPKVD NNAIEFLLLQ ASDGIHHWTP PKGHVEPGED DLETALRETQ EEAGIEAGQL TIIEGFKREL NYVARNKPKT VIYWLAEVKD YDVEIRLSHE HQAYRWLGLE EACQLAQFKE MKAALQEGHQ FLCSIEA|
|Grade & Purity||> 90% pure|
|Molecular Weight||19.0 kDa (167aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NUDT2 is a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. This protein maintains homeostasis by using water to cleave the metabolite NUDT symmetrically back into its original ATP and AMP molecules. NUDT2 is also active towards other adenosine and diadenosine polyphosphates with four or more phosphate groups, but not towards diadenosine triphosphate. NUDT2 is involved in heat shock and metabolic stress by regulating intracellular dinucleoside polyphosphate concentrations. Recombinant NUDT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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