ORC6L antibody (10R-1562)

Mouse monoclonal ORC6L antibody

Synonyms Monoclonal ORC6L antibody, Anti-ORC6L antibody, Origin recognition complex subunit 6 antibody, ORCL 6 antibody, ORCL 6, ORC6L, ORCL-6, ORCL-6 antibody, ORC6 antibody
Specificity Human ORC6L
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen ORC6L antibody was raised in mouse using recombinant Human Origin Recognition Complex, Subunit 6 Like (Yeast) (Orc6L)

Images

Immunofluorescent staining using ORC6L antibody (10R-1562)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ORC6L antibody.

Specifications

Host Mouse
Clone 2679C2b
Isotype IgG1
Method of Purification ORC6L antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer ORC6L antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and and ORC1L are loosely associated with the core complex consisting of ORC2L, -3L, -4L and -5L. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Immunofluorescent staining using ORC6L antibody (10R-1562) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ORC6L antibody.
  • Western Blot analysis of immunized recombinant protein using ORC6L antibody (10R-1562)

Availability: In stock

Price: €157.01
Size: 50 ug
OR
Shipping
View Our Distributors