OTUB1 antibody (70R-12629)

Affinity purified Rabbit polyclonal OTUB1 antibody

Synonyms Polyclonal OTUB1 antibody, Anti-OTUB1 antibody, MGC111158 antibody, ubiquitin-specific-processing protease OTUB1 antibody, otubain-1 antibody, OTUB-1 antibody, ubiquitin thioesterase OTUB1 antibody, OTUB1 antibody, OTUB 1 antibody, FLJ20113 antibody, deubiquitinating enzyme OTUB1 antibody, ubiquitin-specific protease otubain 1 antibody, MGC4584 antibody, OTU1 antibody, OTUB 1, OTUB1, FLJ40710 antibody, OTUB-1, OTB1 antibody, OTU domain-containing ubiquitin aldehyde-binding protein 1 antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, WB
Immunogen OTUB1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 149 of OTUB1

Images

Western blot analysis using OTUB1 antibody (70R-12629)

Western blot analysis of 50 ug of whole cell lysate (A: Mouse liver) using a 12 % SDS PAGE gel and OTUB1 antibody at a dilution of 1:500

Specifications

Host Rabbit
Isotype IgG
Method of Purification OTUB1 antibody was purified by antigen-affinity chromatography
Molecular Weight 31 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using OTUB1 antibody (70R-12629)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse liver) using a 12 % SDS PAGE gel and OTUB1 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using OTUB1 antibody (70R-12629)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hela; B: Hep G2) using a 12 % SDS PAGE gel and OTUB1 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using OTUB1 antibody (70R-12629)</font></div>     | Immunofluorescence analysis of methanol-fixed HeLa, using OTUB1 antibody at 1:50 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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