p27 antibody (20-PR78)

Rabbit polyclonal p27 antibody

Synonyms Polyclonal p27 antibody, Anti-p27 antibody, Cyclin-dependent kinase inhibitor p27 antibody, p -27, p -27 antibody, p27, Cyclin-dependent kinase inhibitor 1B antibody, p27Kip1 antibody, p 27, p 27 antibody
Specificity Human p27
Cross Reactivity To be determined by end-user
Applications ELISA, IP, WB
Immunogen p27 antibody was raised in rabbit using a full length recombinant human p27 protein as the immunogen.

Images

Western Blot analysis using p27 antibody (20-PR78)

Western blot using affinity purified p27 antibody shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead).

Specifications

Host Rabbit
Method of Purification p27 antibody was purified by delipidation and defibrination.
Form & Buffer 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
Concentration 80 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:40,000, IP: 1:100, WB: 1:1,000-1:4,000

Storage & Safety

Storage Store at -20 deg C. Aliquot and freeze at -20 deg C or below for long term storage. This product is stable for several weeks at 4 deg C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance p27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

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Images

  • Western Blot analysis using p27 antibody (20-PR78) | Western blot using affinity purified p27 antibody shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead).

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