p38 antibody (70R-51505)

Purified Polyclonal p38 antibody

Synonyms Polyclonal p38 antibody, Anti-p38 antibody, CSBP antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, MXI2 antibody, SAPK2A antibody, Mitogen-activated protein kinase 14 antibody, MAP kinase 14 antibody, MAPK 14 antibody, Cytokine suppressive anti-inflammatory drug-binding protein antibody, CSAID-binding protein antibody, CSBP antibody, MAP kinase MXI2 antibody, MAX-interacting protein 2 antibody, Mitogen-activated protein kinase p38 alpha antibody, MAP kinase p38 alpha antibody, Stress-activated protein kinase 2a antibody, SAPK2a antibody
Specificity Recognizes endogenous levels of p38 protein
Cross Reactivity Mouse, Rat, Dog
Applications ICC, IF, IHC, WB
Immunogen p38 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p38 as the immunogen

Images

Western blot analysis using p38 antibody (70R-51505)

Western blot analysis of p38 expression in HeLa (A); Raw264.7 (B); H9C2 (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification p38 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance p38 is the mammalian orthologue of the yeast Hog1p MAP kinase, which participates in a signaling cascade controlling cellular responses to cytokines and stress.

References
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Images

  • 

Western blot analysis using p38 antibody (70R-51505)



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Western blot analysis of p38 expression in HeLa (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
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Immunofluorescent analysis using p38 antibody (70R-51505)



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Immunofluorescent analysis of p38 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Immunohistochemical analysis using p38 antibody (70R-51505)



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Immunohistochemical analysis of p38 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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