p53 antibody (20R-1957)

Rabbit polyclonal p53 antibody

Synonyms Polyclonal p53 antibody, Anti-p53 antibody, p-53 antibody, p53, p-53, p53 Oncoprotein antibody, p 53, Phospho Ser315 antibody, p 53 antibody, BCC7 antibody, BOS_18908 antibody, brp53 antibody, cellular tumor antigen p53 antibody, drp53 antibody, EGK_08142 antibody, etID22686.5 antibody, fb40d06 antibody, LFS1 antibody, Li-Fraumeni syndrome antibody, p53 antibody, p53 tumor suppressor antibody, p53 tumor suppressor phosphoprotein antibody, phosphoprotein p53 antibody, transformation related protein 53 antibody, transformation-related protein 53 antibody, trp53 antibody, tumor protein 53 antibody, tumor protein p53 (Li-Fraumeni syndrome) antibody, tumor suppressing protein antibody, tumor suppressor homolog p53 antibody, Tumor suppressor p53 antibody, tumor suppressor protein antibody, tumor suppressor protein p53 antibody, tumor suppressor TP53 antibody, tumor supressor p53 antibody, Xp53 antibody
Cross Reactivity Human
Applications IF, IHC, WB
Immunogen p53 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 315 (S-S-S(p)-P-Q) derived from Human p53 as the immunogen.


Immunofluorescent staining using p53 antibody (20R-1957)

Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser315) antibody


Host Rabbit
Method of Purification p53 antibody was purified by immunoaffinity chromatography.
Form & Buffer Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:100-1:200, IHC: 1:50-1:100, WB: 1:500-1:1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance p53 (aka TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitn ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in p53 binding to gene specific response elements.

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  • Immunofluorescent staining using p53 antibody (20R-1957) | Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser315) antibody
  • Western Blot analysis using p53 antibody (20R-1957) | Western Blot analysis of extracts from Hela cells using p53 (Phospho-Ser315) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane 1)
  • Immunohistochemical staining using p53 antibody (20R-1957) | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser315) antibody (left) or the same antibody preincubated with with blocking peptide (right)

Availability: In stock

Price: €185.94
Size: 50 ug
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