p53 antibody (70R-37525)

Rabbit Polyclonal p53 antibody

Synonyms Polyclonal p53 antibody, Anti-p53 antibody, tumor protein p53 antibody, Tumor suppressor p53 antibody, Antigen NY-CO-13 antibody, Cellular tumor antigen p53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, p53 antigen antibody, p53 transformation suppressor antibody, p53 tumor suppressor antibody, Phosphoprotein p53 antibody, TP53 antibody, transformation-related protein 53 antibody, TRP53 antibody
Specificity p53 antibody detects endogenous levels of total p53 protein
Applications IF, IHC, WB
Immunogen p53 antibody was raised in Rabbit using a synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine15 (P-L-SP-Q-E) as the immunogen.

Images

Western blot analysis using p53 antibody (70R-37525)

Western blot analysis of the extracts from HeLa cells untreated or treated with hydroxyurea using p53 antibody and p53 antibody (Lanes 3 and 4)

Specifications

Host Rabbit
Isotype IgG
Method of Purification p53 antibody was purified by affinity chromatography
Form & Buffer Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB:1:500-1000, IHC: 1:50-1:100, IF: 1:100-200

Storage & Safety

Storage Store at -20 deg C in small aliquots. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Activated p53 binds DNA and activates expression of several genes including microRNA miR-34a, WAF1/CIP1 encoding for p21 and hundreds of other down-stream genes. p21 (WAF1) binds to the G1-S/CDK (CDK2) and S/CDK complexes (molecules important for the G1/S transition in the cell cycle) inhibiting their activity.

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Images

  • Western blot analysis using p53 antibody (70R-37525) | Western blot analysis of the extracts from HeLa cells untreated or treated with hydroxyurea using p53 antibody and p53 antibody (Lanes 3 and 4)
  • Immunohistochemical staining using p53 antibody (70R-37525) | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 antibody

Availability: In stock

Price: €203.68
Size: 100 ug
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