p53 antibody (70R-37526)
Rabbit Polyclonal p53 antibody
Overview
Overview
| Synonyms | Polyclonal p53 antibody, Anti-p53 antibody, p53 antigen antibody, p53 transformation suppressor antibody, p53 tumor suppressor antibody, Phosphoprotein p53 antibody, TP53 antibody, transformation-related protein 53 antibody, TRP53 antibody, tumor protein p53 antibody, Tumor suppressor p53 antibody, Antigen NY-CO-13 antibody, Cellular tumor antigen p53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody |
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| Specificity | p53 antibody detects endogenous levels of total p53 protein |
| Applications | WB |
| Immunogen | p53 antibody was raised in Rabbit using a synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of threonine 18 (Q-E-TP-F-S) as the immunogen. |
Images
Western blot analysis using p53 antibody (70R-37526)
Western blot analysis of extracts from HT-29 cells untreated or treated with hydroxyurea, using p53 antibody and p53 antibody (Lanes 3 and 4)
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | p53 antibody was purified by affinity chromatography |
| Form & Buffer | Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB:1:500-1000 |
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Storage & Safety
| Storage | Store at -20 deg C in small aliquots. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | p53 becomes activated in response to myriad stressors, including but not limited to DNA damage (induced by either UV, IR, or chemical agents such as hydrogen peroxide), oxidative stress, osmotic shock, ribonucleotide depletion, and deregulated oncogene expression. |
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