p53 antibody (70R-37529)

Rabbit Polyclonal p53 antibody

Synonyms Polyclonal p53 antibody, Anti-p53 antibody, Phosphoprotein p53 antibody, TP53 antibody, transformation-related protein 53 antibody, TRP53 antibody, tumor protein p53 antibody, Tumor suppressor p53 antibody, Antigen NY-CO-13 antibody, Cellular tumor antigen p53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, p53 antigen antibody, p53 transformation suppressor antibody, p53 tumor suppressor antibody
Specificity p53 antibody detects endogenous levels of total p53 protein
Applications WB
Immunogen p53 antibody was raised in Rabbit using a synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 37 (L-P-SP-Q-A) as the immunogen.

Images

Western blot analysis using p53 antibody (70R-37529)

Western blot analysis of extract from HT-29 cell untreated or treated with Doxorubicin (1mM, 30min), using p53 antibody and p53 antibody (Lanes 3 and 4)

Specifications

Host Rabbit
Isotype IgG
Method of Purification p53 antibody was purified by affinity chromatography
Form & Buffer Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:1000

Storage & Safety

Storage Store at -20 deg C in small aliquots. Avoid repeat freeze/thaw cycles

General Information

Biological Significance In the cell, p53 protein binds DNA, which in turn stimulates another gene to produce a protein called p21 that interacts with a cell division-stimulating protein (cdk2). When p21 is complexed with cdk2 the cell cannot pass through to the next stage of cell division.

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Images

  • Western blot analysis using p53 antibody (70R-37529) | Western blot analysis of extract from HT-29 cell untreated or treated with Doxorubicin (1mM, 30min), using p53 antibody and p53 antibody (Lanes 3 and 4)

Availability: In stock

Price: €203.68
Size: 100 ug
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