p53 antibody (70R-37529)
Rabbit Polyclonal p53 antibody
Overview
Overview
| Synonyms | Polyclonal p53 antibody, Anti-p53 antibody, Phosphoprotein p53 antibody, TP53 antibody, transformation-related protein 53 antibody, TRP53 antibody, tumor protein p53 antibody, Tumor suppressor p53 antibody, Antigen NY-CO-13 antibody, Cellular tumor antigen p53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, p53 antigen antibody, p53 transformation suppressor antibody, p53 tumor suppressor antibody |
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| Specificity | p53 antibody detects endogenous levels of total p53 protein |
| Applications | WB |
| Immunogen | p53 antibody was raised in Rabbit using a synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 37 (L-P-SP-Q-A) as the immunogen. |
Images
Western blot analysis using p53 antibody (70R-37529)
Western blot analysis of extract from HT-29 cell untreated or treated with Doxorubicin (1mM, 30min), using p53 antibody and p53 antibody (Lanes 3 and 4)
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | p53 antibody was purified by affinity chromatography |
| Form & Buffer | Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB: 1:500-1:1000 |
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Storage & Safety
| Storage | Store at -20 deg C in small aliquots. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | In the cell, p53 protein binds DNA, which in turn stimulates another gene to produce a protein called p21 that interacts with a cell division-stimulating protein (cdk2). When p21 is complexed with cdk2 the cell cannot pass through to the next stage of cell division. |
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