p53 antibody (70R-51665)
Purified Polyclonal p53 antibody
Overview
Overview
| Synonyms | Polyclonal p53 antibody, Anti-p53 antibody, P53 antibody, Cellular tumor antigen p53 antibody, Antigen NY-CO-13 antibody, Phosphoprotein p53 antibody, Tumor suppressor p53 antibody |
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| Specificity | Recognizes endogenous levels of p53 protein |
| Applications | IP, WB |
| Immunogen | p53 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p53 as the immunogen |
Images
Western blot analysis using p53 antibody (70R-51665)
Western blot analysis of p53 expression in THP1 (A); Jurkat (B) whole cell lysates.
Specifications
| Host | Rabbit |
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| Method of Purification | p53 antibody was purified by immunogen affinity chromatography |
| Form & Buffer | Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide |
Usage & Assay Information
| Usage Recommendations | WB: 1:500 - 1:1000, IP: 1:10 - 1:100 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | p53 is a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. |
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