1:50 for 30 minutes at 25 deg C. The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections.
Storage & Safety
Storage
Store at 4 deg C. DO NOT FREEZE. Return to 4 deg Cimmediately after use.
General Information
Product Use
p57 antibody is for use in immunohistochemistry on paraffin sections
Biological Significance
p57/Kip2 is also known as Cyclin-dependent kinase inhibitor 1C(CDKN1C). p57/Kip2 is a tight-binding inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation.
Implications in Disease
Mutations of the CDKN1C gene which encodes p57, are implicated in sporadic cancers and Beckwith-Wiedemann syndrome suggesting that it is a tumor suppressor candidate.