PAFAH protein (30R-AP045)

Purified recombinant Human PAFAH protein

Synonyms PAF 2-acylhydrolase protein, PAF acetylhydrolase protein, LDL-associated phospholipase A2 protein, LDL-PLA2 protein, 2-acetyl-1-alkylglycerophosphocholine esterase protein, 1-alkyl-2-acetylglycerophosphocholine esterase protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of PAFAH protein corresponding to amino acids FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN.
Expression System 293 cells
Grade & Purity > 95% pure
Molecular Weight 47-55 kDa
Form & Buffer Supplied as a lyophilized powder.

Usage & Assay Information

Bioactivity Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF-AH concentration of 10.0 ug/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the ?sn2? ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids.

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Availability: In stock

Price: €211.69
Size: 20 ug
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