PAK1 antibody (70R-11714)

Rabbit polyclonal PAK1 antibody

Synonyms Polyclonal PAK1 antibody, anti-PAK1 antibody, Rac1-activated kinase 1 antibody, PAK1, PAK 1, PAK-1 antibody, Cdc42 antibody, alpha-PAK antibody, PAK1 antibody, PAK alpha antibody, p65-PAK antibody, MGC130000 antibody, PAK 1 antibody, PAK-1 antibody, p21 antibody, MGC130001 antibody, PAK-1
Cross Reactivity Human
Applications IP, WB
Immunogen PAK1 antibody was raised in Rabbit using recombinant human PAK1 as the immunogen.

Images

Western Blot analysis using PAK1 antibody (70R-11714)

Western blot analysis of PAK1 with transfected HEK293 cells.

Specifications

Host Rabbit
Isotype IgG
Method of Purification PAK1 antibody was purified by affinity chromatography
Form & Buffer Supplied in PBS buffer, pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations IP: 10-40 ug/ml, IHC: 10-40 ug/ml, WB: 0.5-4 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PAK1 is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase, PAK1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK1 inhibits myosin light chain kinase, and appears to play a role in the regulation of apoptosis by phosphorylating the death agonist Bad. Membrane targeting of PAK1 induces neurite outgrowth. PAK1 may also regulate NFkB activation.

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Images

  • <div><font face=Calibri size=2>Western Blot analysis using PAK1 antibody (70R-11714)</font></div>     | Western blot analysis of PAK1 with transfected HEK293 cells.

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