PCCB antibody (70R-12884)

Affinity purified Rabbit polyclonal PCCB antibody

Synonyms Polyclonal PCCB antibody, Anti-PCCB antibody, propionyl Coenzyme A carboxylase beta polypeptide antibody, PCCase subunit beta antibody, propanoyl-CoA:carbon dioxide ligase subunit beta antibody, PCCB antibody, DKFZp451E113 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen PCCB antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 167 and 480 of PCCB

Images

Immunofluorescent staining using PCCB antibody (70R-12884)

Immunofluorescence analysis of methanol-fixed Hep G2, using PCCB antibody at 1:500 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification PCCB antibody was purified by antigen-affinity chromatography
Molecular Weight 58 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.54 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using PCCB antibody (70R-12884)</font></div>     | Immunofluorescence analysis of methanol-fixed Hep G2, using PCCB antibody at 1:500 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using PCCB antibody (70R-12884)</font></div>     | Immunohistochemical staining of paraffin-embedded Lung Ad Cancer PC14 xenograft using PCCB antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using PCCB antibody (70R-12884)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A: Hep G2) using a 7.5 % SDS PAGE gel and PCCB antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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