PCNA antibody (10R-1089)

Mouse monoclonal PCNA antibody

Synonyms Monoclonal PCNA antibody, Anti-PCNA antibody, Proliferating cell nuclear antigen antibody, Proliferating cell nuclear antigen antibody, MGC8367 antibody, Proliferating cell nuclear antigen antibody, Anti PCNA antibody , Proliferating cell nuclear antigen antibody, PCNA antibody, PCNA antibody, PCNA antibody, MGC8367 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, WB
Immunogen PCNA antibody was raised in mouse using recombinant human PCNA (1-261aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of PCNA antibody (10R-1089)

Cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human PCNA (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone k1A3
Isotype IgG3 kappa
Source Anti-human PCNA antibody, clone k1A3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PCNA protein.
Method of Purification PCNA antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1,000-1:3,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors.

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Images

  • Western Blot analysis of PCNA antibody (10R-1089) | Cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human PCNA (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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