Immunohistochemical staining using PCNA antibody (biotin) (61R-P152aBT)
Formalin-fixed, paraffin-embedded human small intestine stained with PCNA antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of proliferating cell
PCNA antibody (biotin)
Immunohistochemical staining using PCNA antibody (biotin) (61R-P152aBT)
Formalin-fixed, paraffin-embedded human small intestine stained with PCNA antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of proliferating cell
Immunohistochemical staining using PCNA antibody (biotin) (61R-P152aBT)
Formalin-fixed, paraffin-embedded rat breast cancer stained with PCNA antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
Specifications
Host
Mouse
Clone
PC10
Isotype
IgG2a kappa
Method of Purification
PCNA antibody (biotin) was purified by Protein G chromatography.
Molecular Weight
36 kDa (predicted detection band MW)
Tag/Conjugate
Biotin
Form & Buffer
Purified from ascites fluid by protein G chromatography and supplied in 10mM PBS, pH 7.4.
Concentration
Batch dependent - please inquire should you have specific requirements
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
General Information
Biological Significance
Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. Its level correlates directly with rates of cellular proliferation and DNA synthesis. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation.