PCNA protein (30R-1156)
Purified recombinant Human PCNA protein
Overview
Overview
| Synonyms | PCNA protein, , Proliferating cell nuclear protein protein, MGC8367 protein, Proliferating cell nuclear protein protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of PCNA protein (30R-1156)
Figure annotation denotes ug of protein loaded and % gel used.
PCNA protein
Coomassie Blue stained SDS-PAGE of PCNA protein (30R-1156)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-261 amino acids: MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 28 kDa (261 aa), confirmed by MALDI-TOF. |
| Form & Buffer | Supplied as a liquid in 20mM Tris, pH 7.5, containing 2mM EDTA, and 20% glycerol. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | PCNA (Proliferating Cell Nuclear protein) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E. coli and purified by conventional chromatography techniques. |
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