PDAP1 protein (His tag) (80R-1420)
Purified recombinant Human PDAP1 protein
|Synonyms||PDAP-1 protein, HASPP28 protein, PAP protein, PDAP 1 protein, PDGFA associated protein 1, PDAP 1, PDAP-1, PAP1 protein, PDAP1|
Coomassie Blue stained SDS-PAGE of PDAP1 protein (His tag) (80R-1420)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-181 amino acids: MPKGGRKGGH KGRARQYTSP EEIDAQLQAE KQKAREEEEQ KEGGDGAAGD PKKEKKSLDS DESEDEEDDY QQKRKGVEGL IDIENPNRVA QTTKKVTQLD LDGPKELSRR EREEIEKQKA KERYMKMHLA GKTEQAKADL ARLAIIRKQR EEAARKKEEE RKAKDDATLS GKRMQSLSLN KLEHHHHHH|
|Grade & Purity||> 90% pure|
|Molecular Weight||21.7 kDa (189aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 2mM DTT, 100mM NaCl and 0.1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PDGFA associated protein 1, also known as PDAP1, enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB. And its expression is induced by TNF-alpha, and cells overexpressing PDAP1 show significantly less apoptosis on exposure to TNF-alpha. Recombinant human PDAP1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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