PDGFAA protein (Mouse) (30R-2235)

Purified recombinant Mouse PDGFAA protein

Synonyms Platelet Derived Growth Factor AA protein, PDGF-1 protein
Specificity Human
Species Mouse
Protein Type Recombinant
Applications Functional Assay
Assay Information The recombinant mouse IFN-a2 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED50 for this effect is typically 0.1 ng/ml, corresponding to a specific activity of 1 x 107 U/mg.

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Functional Assay using PDGFAA protein (Mouse) (30R-2235)

Proliferation of 3T3 cells in response to Mouse PDGF-AA protein

Specifications

Expression System E.coli
Method of Purification PDGFAA protein was purified by affinity chromatography.
Form & Buffer Supplied as a sterile liquid with phosphate bufferedsaline, 1 mM DTT, 1% BSA, pH 7.2. 0.22 umfiltered.

Usage & Assay Information

Bioactivity The ED50 of this protein, asmeasured by balb/c-3T3 cell proliferation, is0.1-1.25 ng/ml. This corresponds to a specific activity of 1 x 107 - 8 x 105 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance The platelet-derived growth factor AA is a member of the PDGF family. The PDGF proteins are derived from four genes that form four disulfide-linked homodimers and one heterodimer. PDGF-AA plays an important role as a mitogen for a number of cell types. Recombinant Mouse PDGF-AA produced in e.coli is a non-glycosylated homodimer of 126 amino acids with a molecular weight of 28.9 kDa.

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  • Functional Assay using PDGFAA protein (Mouse) (30R-2235) | Proliferation of 3T3 cells in response to Mouse PDGF-AA protein

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