PDGFAA protein (Mouse) (30R-2235)
Purified recombinant Mouse PDGFAA protein
Overview
Overview
| Synonyms | Platelet Derived Growth Factor AA protein, PDGF-1 protein |
|---|---|
| Specificity | Human |
| Species | Mouse |
| Protein Type | Recombinant |
| Applications | Functional Assay |
| Assay Information | The recombinant mouse IFN-a2 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED50 for this effect is typically 0.1 ng/ml, corresponding to a specific activity of 1 x 107 U/mg. |
Images
Functional Assay using PDGFAA protein (Mouse) (30R-2235)
Proliferation of 3T3 cells in response to Mouse PDGF-AA protein
Specifications
| Expression System | E.coli |
|---|---|
| Method of Purification | PDGFAA protein was purified by affinity chromatography. |
| Form & Buffer | Supplied as a sterile liquid with phosphate bufferedsaline, 1 mM DTT, 1% BSA, pH 7.2. 0.22 umfiltered. |
Usage & Assay Information
| Bioactivity | The ED50 of this protein, asmeasured by balb/c-3T3 cell proliferation, is0.1-1.25 ng/ml. This corresponds to a specific activity of 1 x 107 - 8 x 105 Units/mg. |
|---|
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | The platelet-derived growth factor AA is a member of the PDGF family. The PDGF proteins are derived from four genes that form four disulfide-linked homodimers and one heterodimer. PDGF-AA plays an important role as a mitogen for a number of cell types. Recombinant Mouse PDGF-AA produced in e.coli is a non-glycosylated homodimer of 126 amino acids with a molecular weight of 28.9 kDa. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product