PDK1 protein (His tag) (80R-1103)

Purified recombinant Human PDK1 protein

Synonyms Mitochondrial pyruvate dehydrogenase kinase isoenzyme1 protein, isoenzyme 1., Pyruvate dehydrogenase kinase protein, PDK1, PDK 1 protein, Pyruvate dehydrogenase kinase protein, isozyme 1 protein, PDK-1 protein, isozyme 1 HGNC:8809 protein, PDK 1, Pyruvate dehydrogenase kinase protein, PDK1 protein, PDK-1, Pyruvate dehydrogenase kinase isoform 1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PDK1 protein (His tag) (80R-1103)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 29-436 amino acids: MGSSHHHHHH SSGLVPRGSH MSSDSGSSPA SERGVPGQVD FYARFSPSPL SMKQFLDFGS VNACEKTSFM FLRQELPVRL ANIMKEISLL PDNLLRTPSV QLVQSWYIQS LQELLDFKDK SAEDAKAIYD FTDTVIRIRN RHNDVIPTMA QGVIEYKESF GVDPVTSQNV QYFLDRFYMS RISIRMLLNQ HSLLFGGKGK GSPSHRKHIG SINPNCNVLE VIKDGYENAR RLCDLYYINS PELELEELNA KSPGQPIQVV YVPSHLYHMV FELFKNAMRA TMEHHANRGV YPPIQVHVTL GNEDLTVKMS DRGGGVPLRK IDRLFNYMYS TAPRPRVETS RAVPLAGFGY GLPISRLYAQ YFQGDLKLYS LEGYGTDAVI YIKALSTDSI ERLPVYNKAA WKHYNTNHEA DDWCVPSREP KDMTTFRSA
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 48.6 kDa (429 aa)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.0, containing 100mM NaCl, 0.5mM DTT, 0.1mM EDTA, 0.1mM PMSF, 1mM MgCl2, and 40% glycerol.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth. Recombinant human PDK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of PDK1 protein (His tag) (80R-1103) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €169.35
Size: 100 ug
OR
Shipping
View Our Distributors