PE2 antibody (70R-49717)

Purified Polyclonal PE2 antibody

Synonyms Polyclonal PE2 antibody, Anti-PE2 antibody, ETS domain-containing transcription factor ERF antibody, Ets2 repressor factor antibody, PE-2 antibody
Specificity Recognizes endogenous levels of PE2 protein
Cross Reactivity Mouse
Applications ICC, IF, IHC, WB
Immunogen PE2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PE2 as the immunogen

Images

Western blot analysis using PE2 antibody (70R-49717)

Western blot analysis of PE2 expression in A431 (A); HeLa (B); NIH3T3 (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification PE2 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance PE2 is a potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation.

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Images

  • 

Western blot analysis using PE2 antibody (70R-49717)



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Western blot analysis of PE2 expression in A431 (A); HeLa (B); NIH3T3 (C) whole cell lysates.
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Immunofluorescent analysis using PE2 antibody (70R-49717)



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Immunofluorescent analysis of PE2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Immunohistochemical analysis using PE2 antibody (70R-49717)



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Immunohistochemical analysis of PE2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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