PEX11B antibody (70R-50642)

Purified Polyclonal PEX11B antibody

Synonyms Polyclonal PEX11B antibody, Anti-PEX11B antibody, Peroxisomal membrane protein 11B antibody, Peroxin-11B antibody, Peroxisomal biogenesis factor 11B antibody, Protein PEX11 homolog beta antibody, PEX11-beta antibody
Specificity Recognizes endogenous levels of PEX11B protein
Cross Reactivity Mouse, Rat, Bovine
Applications ICC, IF, IHC, IP, WB
Immunogen PEX11B antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PEX11B as the immunogen

Images

Western blot analysis using PEX11B antibody (70R-50642)

Western blot analysis of PEX11B expression in HT29 (A); THP1 (B); Jurkat (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification PEX11B antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance PEX11B facilitates peroxisomal proliferation and interacts with PEX19. The encoded protein is found in the peroxisomal membrane.

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Images

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Western blot analysis using PEX11B antibody (70R-50642)



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Western blot analysis of PEX11B expression in HT29 (A); THP1 (B); Jurkat (C) whole cell lysates.
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Immunohistochemical analysis using PEX11B antibody (70R-50642)



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Immunohistochemical analysis of PEX11B staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using PEX11B antibody (70R-50642)



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Immunofluorescent analysis of PEX11B staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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