PFDN2 protein (His tag) (80R-1535)
Purified recombinant Human PFDN2 protein
|Synonyms||PFDN-2, PFDN2, PFDN 2 protein, Prefoldin subunit 2 protein, HSPC231, PFDN 2, PFDN-2 protein, PFD2 protein|
Coomassie Blue stained SDS-PAGE of PFDN2 protein (His tag) (80R-1535)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-154 amino acids: MGSSHHHHHH SSGLVPRGSH MAENSGRAGK SSGSGAGKGA VSAEQVIAGF NRLRQEQRGL ASKAAELEME LNEHSLVIDT LKEVDETRKC YRMVGGVLVE RTVKEVLPAL ENNKEQIQKI IETLTQQLQA KGKELNEFRE KHNIRLMGED EKPAAKENSE GAGAKASSAG VLVS|
|Grade & Purity||> 90% pure|
|Molecular Weight||18.8 kDa (174aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 50mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PFDN2 (Prefoldin subunit 2) is a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. This protein binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. And binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Recombinant human PFDN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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