PFDN6 protein (His tag) (80R-1724)
Purified recombinant Human PFDN6 protein
|Synonyms||KE-2 protein, MGC70744 protein, PFD6 protein, HKE2 protein, PFDN6, PFDN 6 protein, H2-KE2 protein, Prefoldin subunit 6 protein, PFDN 6, PFDN-6 protein, PFDN-6|
Coomassie Blue stained SDS-PAGE of PFDN6 protein (His tag) (80R-1724)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-129 amino acids: MGSSHHHHHH SSGLVPRGSH MAELIQKKLQ GEVEKYQQLQ KDLSKSMSGR QKLEAQLTEN NIVKEELALL DGSNVVFKLL GPVLVKQELG EARATVGKRL DYITAEIKRY ESQLRDLERQ SEQQRETLAQ LQQEFQRAQA AKAGAPGKA|
|Grade & Purity||> 95% pure|
|Molecular Weight||16.7 kDa (149aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.1M NaCl, 1mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PFDN6, also known as HKE2 or PFD6, is a subunit of the heteromeric prefoldin complex that chaperones nascent actin and alpha- and beta-tubulin chains pending their transfer to the cytosolic chaperonin containing TCP1 (CCT) complex. It binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it and binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Recombinant human PFDN6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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