PFKM protein (His tag) (80R-1301)
Purified recombinant Human PFKM protein
Overview
Overview
| Synonyms | GSD7 protein, Phosphohexokinase., Phosphofructokinase M protein, EC 2.7.1.11 protein, 6-Phosphofructokinase protein, Phosphofructokinase protein, Phosphofructokinase protein, PFK1 protein, PFKL protein, polypeptide X protein, muscle protein, MGC8699 protein, muscle type protein, PFKA protein, PFKX protein, PFKP protein, PFKM protein, Phosphofructokinase protein, PFK-1 protein, Phosphofructo 1 Kinase Isozyme A protein, Phosphofructokinase 1 protein, muscle type 6 Phosphofructokinase Muscle Type protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of PFKM protein (His tag) (80R-1301)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-780 amino acids: MGSSHHHHHH SSGLVPRGSH MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD GGDHIKEATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAYNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLSDLQ KAGKITDEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIMEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK AMDEKKFDEA LKLRGRSFMN NWEVYKLLAH VRPPVSKSGS HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKSFEQ ISANITKFNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSV GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF TIRDLQANVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF QPVAELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 80% pure |
| Molecular Weight | 87.3 kDa (800aa), confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 5mM DTT, 0.2 M NaCl and 20% glycerol. |
| Concentration | 0.5 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
|---|
General Information
| Biological Significance | PFKM is a regulatory glycolytic enzyme that converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Recombinant human PFKM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product