Pfn1 protein (80R-4318)
Purified Recombinant Pfn1 protein (His tagged)
Overview
Overview
| Synonyms | PFN1 protein, PROF1 protein, profilin 1 protein, Profilin I protein, Profilin-1 protein |
|---|---|
| Species | Rat |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
SDS-PAGE analysis using Pfn1 protein (His tag) (80R-4318)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-140 aa: MGSSHHHHHH SSGLVPRGSH MGSHMAGWNA YIDSLMADGT CQDAAIVGYK DSPSVWAAVP GKTFVSITPA EVGVLVGKDR SSFFVNGLTL GGQKCSVIRD SLLQDGEFTM DLRTKSTGGA PTFNVTVTMT AKTLVLLMGK EGVHGGLINK KCYEMASHLR RSQY |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 17.5 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in PBS buffer(pH 7.4) containing 10% glycerol1mM DTT |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage. For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | Pfn1 also known as Profilin-1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals. Recombinant rat Pfn1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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