PGD antibody (70R-12600)

Affinity purified Rabbit polyclonal PGD antibody

Synonyms Polyclonal PGD antibody, Anti-PGD antibody, PGD antibody, 6PGD antibody, 6-phosphogluconate dehydrogenase decarboxylating antibody, phosphogluconate dehydrogenase antibody
Cross Reactivity Human
Applications ICC, IF, WB
Immunogen PGD antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 201 of PGD

Images

Immunofluorescent staining using PGD antibody (70R-12600)

Immunofluorescence analysis of methanol-fixed HeLa, using PGD antibody at 1:100 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification PGD antibody was purified by antigen-affinity chromatography
Molecular Weight 53 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance 6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using PGD antibody (70R-12600)</font></div>     | Immunofluorescence analysis of methanol-fixed HeLa, using PGD antibody at 1:100 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using PGD antibody (70R-12600)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:293T; B:MOLT4; C:Raji) using a 10 % SDS PAGE gel and PGD antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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