PGP 9.5 Blocking Peptide (33R-10434)
A synthetic peptide for use as a blocking control in assays to test for specificity of PGP 9.5 antibody, catalog no. 20R-PG011
Overview
Overview
| Synonyms | Ubiquitin carboxyl-terminal esterase L1 Blocking Peptide, Neuron cytoplasmic protein 9.5 Blocking Peptide, Ubiquitin thioesterase L1 Blocking Peptide, PARK5 Blocking Peptide, Ubiquitin C-terminal hydrolase Blocking Peptide, UCH-L1 Blocking Peptide |
|---|---|
| Protein Type | Synthetic |
| Applications | User optimized |
| Assay Information | Antibody should be pre-incubated with peptide prior to incubation with tissue/sample. We suggest perincubation for 1 hour at room temperature. A 10^-6 or 10^-5 molar (M) peptide concentration is suggested to block antigen/antibody binding. However, optimal working concentration should be determined by the investigator using a range of peptide dilutions to eliminate or reduce immunoreactivity. |
Specifications
| Residues | GASSEDTLLKDAAKVCR |
|---|---|
| Molecular Weight | 1.763 kDa |
| Form & Buffer | Supplied in lyophilized form. Reconstitute with deionized water. |
Usage & Assay Information
| Usage Recommendations | 2mgs/ml is recommended for most absorption control applications. |
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Storage & Safety
| Storage | Aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | This enzyme is a thiol protease that hydrolyzes apeptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cellsof the diffuse neuroendocrine system. |
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