PHLP antibody (70R-13060)

Affinity purified Rabbit polyclonal PHLP antibody

Synonyms Polyclonal PHLP antibody, Anti-PHLP antibody, phosducin-like antibody, PDCL antibody, DKFZp564M1863 antibody, PhLP antibody, phosducin-like protein antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, WB
Immunogen PHLP antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 204 of PHLP

Images

Western blot analysis using PHLP antibody (70R-13060)

Western blot analysis of 50 ug of whole cell lysate (A: mouse liver) using a 10 % SDS PAGE gel and PHLP antibody at a dilution of 1:2000

Specifications

Host Rabbit
Isotype IgG
Method of Purification PHLP antibody was purified by antigen-affinity chromatography
Molecular Weight 34 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Phosducin-like protein is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using PHLP antibody (70R-13060)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: mouse liver) using a 10 % SDS PAGE gel and PHLP antibody at a dilution of 1:2000
  • <div><font face=Calibri size=2>Western blot analysis using PHLP antibody (70R-13060)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:H1299) using a 10 % SDS PAGE gel and PHLP antibody at a dilution of 1:3000
  • <div><font face=Calibri size=2>Immunofluorescent staining using PHLP antibody (70R-13060)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PHLP antibody at 1:200 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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