phospho EGFR antibody (10R-E125a)

Mouse monoclonal phospho EGFR antibody

Synonyms Monoclonal phospho EGFR antibody, Anti-phospho EGFR antibody, Phosphorylated EGFR antibody, pEGFR antibody, phospho-EGFR antibody, Phosphorylated Epidermal Growth Factor Receptor antibody
Specificity Human
Cross Reactivity Dog, mouse, rat
Applications ELISA, ICC, IP, WB
Immunogen Phospho EGFR antibody was raised in mouse using a sythetic peptide conjugated to KLH with contains the Y1173 motif of the human EGFR as the immunogen.

Images

Western blot analysis using phospho EGFR antibody (10R-E125a)

Untreated A431 and EGF treated A431 lysate were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-phospho-EGF Receptor (1:500 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-EGF protein (~175 kDa).

Specifications

Host Mouse
Clone 9H2
Isotype IgG1 kappa
Method of Purification phospho EGFR antibody was purified by Thiophilic adsorption and size exclusion chromatography.
Molecular Weight 175 kDa (predicted detection band MW)
Form & Buffer Lyophilized from 1ml of PBS, with 0.09% NaN3, PEG, and sucrose. Reconstitute with 0.5 ml of sterile distilled water.

Usage & Assay Information

Usage Recommendations ELISA: 0.05 ug/ml

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze-thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.

General Information

Biological Significance The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGF alpha). EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity.

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Images

  • Western blot analysis using phospho EGFR antibody (10R-E125a) | Untreated A431 and EGF treated A431 lysate were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-phospho-EGF Receptor (1:500 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-EGF protein (~175 kDa).

Availability: In stock

Price: €357.52
Size: 50 ug
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