Phycoerythrin dye (85R-1018)
Phycocyanin dye for use in conjugation to biomolecules and antibodies
Overview
Overview
| Synonyms | Phycoerythrin fluorescent dye, Phycoerythrin, Phycoerythrin fluorescent pigment, R-PE dye, PE dye, PE pigment |
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| Protein Type | Synthetic |
| Applications | Conjugation, FC, IF, Immunoassays |
| Assay Information | Absorption maxima: 565 nm ± 2 nm Emission maxima: 580 nm ± 2 nm Absorbance: A^1% 565 = 82 Quantum Yield = 84.56% |
Specifications
| Source | Porphyra |
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| Molecular Weight | 240 kDa |
| Form & Buffer | Supplied in 100 mM sodium phosphate buffer pH 7.0 with 60 % ammonium sulfate with 0.02 % sodium azide |
Usage & Assay Information
| Usage Recommendations | For conjugation to antibodies and biomolecules for use in RET and HTRF assays, FACS, FISH, fluorescence microscopy and multiple pigments labeled with antibodies for the detection of specific tumors. |
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Storage & Safety
| Storage | Store at 4 deg C. Protect from light. Do not Freeze. |
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General Information
| Biological Significance | Phycoerythrin is a red protein from the light-harvesting phycobiliprotein family, present in cyanobacteria, red algae and cryptomonads. Phycoerythrobilin is the typical chromophore in phycoerythrin. Like all phycobiliproteins, phycoerythrin is composed of a protein part, organised in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. This Phycoerythrin dye has a Quantum Yield = 84.56%, far in excess of existing fluorophores. |
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