Phycoerythrin dye (85R-1018)

Phycocyanin dye for use in conjugation to biomolecules and antibodies

Synonyms Phycoerythrin fluorescent dye, Phycoerythrin, Phycoerythrin fluorescent pigment, R-PE dye, PE dye, PE pigment
Protein Type Synthetic
Applications Conjugation, FC, IF, Immunoassays
Assay Information Absorption maxima: 565 nm ± 2 nm Emission maxima: 580 nm ± 2 nm Absorbance: A^1% 565 = 82 Quantum Yield = 84.56%

Images

Excitation/Emission spectrums showing enhanced quantum yield of Phycoerythrin dye (85R-1018)

Specifications

Source Porphyra
Molecular Weight 240 kDa
Form & Buffer Supplied in 100 mM sodium phosphate buffer pH 7.0 with 60 % ammonium sulfate with 0.02 % sodium azide

Usage & Assay Information

Usage Recommendations For conjugation to antibodies and biomolecules for use in RET and HTRF assays, FACS, FISH, fluorescence microscopy and multiple pigments labeled with antibodies for the detection of specific tumors.

Storage & Safety

Storage Store at 4 deg C. Protect from light. Do not Freeze.

General Information

Biological Significance Phycoerythrin is a red protein from the light-harvesting phycobiliprotein family, present in cyanobacteria, red algae and cryptomonads. Phycoerythrobilin is the typical chromophore in phycoerythrin. Like all phycobiliproteins, phycoerythrin is composed of a protein part, organised in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. This Phycoerythrin dye has a Quantum Yield = 84.56%, far in excess of existing fluorophores.

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Images

  • Excitation/Emission spectrums showing enhanced quantum yield of Phycoerythrin dye (85R-1018)
  • Absorbance and Fluorescence properties of Phycoerythrin dye (85R-1018)

Availability: In stock

Price: €76.07
Size: 1 mg
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