PKA alpha antibody (70R-13088)

Affinity purified Rabbit polyclonal PKA alpha antibody

Synonyms Polyclonal PKA alpha antibody, Anti-PKA alpha antibody, protein kinase A catalytic subunit antibody, PKA C-alpha antibody, PRKACA antibody, MGC48865 antibody, cAMP-dependent protein kinase catalytic subunit alpha isoform 1 antibody, MGC102831 antibody, cAMP-dependent protein kinase catalytic subunit alpha antibody, PKACA antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, WB
Immunogen PKA alpha antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 351 of

Images

Western blot analysis using PKA alpha antibody (70R-13088)

Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: C2C12) using a 10 % SDS PAGE gel and PKA alpha antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification PKA alpha antibody was purified by antigen-affinity chromatography
Molecular Weight 41 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using PKA alpha antibody (70R-13088)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: C2C12) using a 10 % SDS PAGE gel and PKA alpha antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using PKA alpha antibody (70R-13088)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PKA alpha antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
  • <div><font face=Calibri size=2>Western blot analysis using PKA alpha antibody (70R-13088)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3) using a 12 % SDS PAGE gel and PKA alpha antibody at a dilution of 1:500

Availability: In stock

Price: €323.11
Size: 100 ul
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