Western blot analysis using PKA alpha antibody (70R-13088)
Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: C2C12) using a 10 % SDS PAGE gel and PKA alpha antibody at a dilution of 1:1000
PKA alpha antibody
Western blot analysis using PKA alpha antibody (70R-13088)
Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: C2C12) using a 10 % SDS PAGE gel and PKA alpha antibody at a dilution of 1:1000
Immunofluorescent staining using PKA alpha antibody (70R-13088)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PKA alpha antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Western blot analysis using PKA alpha antibody (70R-13088)
Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3) using a 12 % SDS PAGE gel and PKA alpha antibody at a dilution of 1:500
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
PKA alpha antibody was purified by antigen-affinity chromatography
Molecular Weight
41 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase.